By Donald Parriott
This consultant for the working towards chromatographer who wishes a prepared resource of data on HPLC detection explores and compares latest detection structures and detectors, outlines the typical difficulties linked to a given detector, and gives confirmed ways to averting such difficulties.
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Extra info for A Practical Guide to HPLC Detection
005 AUFS on 10-mV recorder. d. Zorbax Rx C-8 column to the detector. A 1-second rise time was used for the ABI detector. 50 Roger Blain compound, ensuring the best possible sensitivity and linearity. Some detectors permit the wavelength to be changed during a Chromatographie run, allowing each compound to be detected at the best possible wavelength. This works only if you don't have a "busy" chromatogram with lots of extra peaks that would interfere with the requisite autozero after a wavelength change.
15% can cause a change in solvent refractive index of 1 x 10" 5 for a 50-50 methanol-water solvent mixture. 8 volume fraction methanol where the slope of the curve in Fig. 2-20 is steeper. It is obvious that premixing the solvents in a single reservoir is much preferred to proportioning the solvents from separate reservoirs by a low pres sure gradient forming pump. C. Change in Solvent Composition with Column Temperature A change in column temperature can affect the baseline of a refractive index detector, as shown in Fig.
C. (1961). Anal. Chem. 33, pp. 286-291. Waters, J. L. (1970). S. Patent 3,535,263 (Nov. 10). Watson, E. S. (1968). S. Patent 3,386,332 (June 4). Yeung, E. S. (1986). " (E. S. ) Wiley, New York, Ch. 1. CHAPTER 3 ABSORBANCE DETECTION Roger Blain Hoffman La Roche Bldg. 86, 8th. floor 340 Kingsland Avenue Nutley, New Jersey 07110 I. OVERVIEW OF ABSORBANCE DETECTORS A major class of HPLC detectors, called absorbance detectors, measures the loss in intensity of ultraviolet or visible light as it passes through the solution exiting an HPLC column.
A Practical Guide to HPLC Detection by Donald Parriott