Download e-book for kindle: Analysis of Carbohydrates by Capillary Electrophoresis by Aran Paulus, Antje Klockow-Beck, Kevin D. Altria

By Aran Paulus, Antje Klockow-Beck, Kevin D. Altria

ISBN-10: 332285020X

ISBN-13: 9783322850201

ISBN-10: 3322850226

ISBN-13: 9783322850225

Carbohydrates are subsequent to nucleic acids and proteins the 3rd very important type of biopolymers and we're simply starting to comprehend their function in organic platforms on a molecular point. lately, the post-translational amendment of proteins has bought elevated curiosity. particularly the glycosylation of enzymes, receptors and antibodies is the point of interest of various examine porjects because it turns into transparent that carbohydrates are concerned about mobile verbal exchange and immuno attractiveness tactics. so as ot comprehend this "sweet sugar language" and accordingly use it in proteomics and the advance of biopharmaceuticals, appropriate analytical options need to be constructed to split carbohydrates with excessive selectivity in complicated organic matrices and quantify them in in minute quantities.

the current monograph covers all features of carbohydrate research through Capillary Electrophoresis together with instrumental facets, derivatization for top sensitivity purposes, separation stipulations and purposes in a number of fields and industries. will probably be a necessary resource of knowledge for everybody occupied with carbohydrate study in lifestyles sciences together with glycobiology, biotechnology and immunology and within the meals industry.

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42 3 Structures and properties of carbohydrates The oligosaccharide moieties can have considerable effects on the bioactivity, the solubility and the stability of proteins, since they are involved in correct folding, and the susceptibility of glycoproteins to proteolysis. Further, these moieties are important in determining their pharmacokinetic profile and their clearance rate. Recognition of oligosaccharide sequences by certain receptors, such as the hepatic Gal/GalNAc-receptor (asialoglycoproteinreceptor) which is located on liver cells, can result in removal of the glycoconjugate or even the whole cell from the circulation.

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Fanali, L. Ossicini, and P. Bocek, "Indirect photometric detection in capillary zone electrophoresis", J. , 470 1986 299. 20. PJ. Oefner, AE. Vorndran, E. Grill, e. K. Bonn, "Capillary zone electrophoretic analysis for carbohydrates by direct and indirect UV detection", Chromatographia, 34 1992 308-316. 21. M. Bruin, AC. van Asten, X. Xu, and H. Poppe, "Theoretical and experimental aspects of indirect detection in capillary electrophoresis", J. , 608 1992 97. 22. Y. J. Dovichi, "Subattomole amino acid analysis by capillary zone electrophoresis and laser-induced fluorescence", Science, 242 1988 562-564.

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Analysis of Carbohydrates by Capillary Electrophoresis by Aran Paulus, Antje Klockow-Beck, Kevin D. Altria

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