Download e-book for iPad: Antigen Processing and Presentation Protocols (Methods in by Solheim J.C.

By Solheim J.C.

ISBN-10: 0896037452

ISBN-13: 9780896037458

ISBN-10: 1592590624

ISBN-13: 9781592590629

Well-recognized and cutting edge experimentalists aspect their state of the art tools for learning the antigen processing and presentation. Drawing on services from biochemistry, mobilephone biology, and immunology, they describe step by step tools designed to query how MHC-binding peptides are generated, to check how peptides are dropped at MHC molecules, to research MHC peptide-binding styles, and to assay the T-cell reaction to the MHC/peptide advanced. Emphasis is given these technical steps serious for experimental good fortune which are usually passed over from tools released within the fundamental literature. Eminently obtainable and state of the art, Antigen Processing and Presentation Protocols offers either new and skilled investigators with hugely useful instruments that may expand the questions that may be requested, and successfully be replied, bearing on antigen processing/presentation.

Show description

Read Online or Download Antigen Processing and Presentation Protocols (Methods in Molecular Biology Vol 156) PDF

Best biology books

Download e-book for kindle: Enzyme-Catalyzed Electron and Radical Transfer by Andreas Holzenburg, Nigel S. Scrutton

Dramatic advances were made in recent times within the box of redox enzymology which has led to a rise of study actions. This quantity will disguise the new milestone advancements during this box through top specialists, uniting idea and scan, and choosing contributions to demonstrate vital features of the mechanisms of electron and radical move in proteins.

Download e-book for kindle: Data Acquisition and Processing in Biology and Medicine. by Kurt Enslein

Info Acquisition and Processing in Biology and medication, quantity five provides the court cases of the 1966 Rochester convention. The ebook provides a version in response to the visco-elastic habit of the aorta and arterioles which can generate aortic strain curves equivalent to genuine ones; and the computer-aided analysis.

Additional info for Antigen Processing and Presentation Protocols (Methods in Molecular Biology Vol 156)

Example text

Luckey, C. , King, G. , Marto, J. , Maier, B. , Crotzer, V. , et al. (1998) Proteasomes can either generate or destroy MHC class I epitopes: evidence for nonproteasomal epitope generation in the cytosol. J. Immunol. 161, 112–121. 33. , McMaster, J. , and Ploegh, H. L. (1998) A proteolytic system that compensates for loss of proteasome function. Nature 392, 618–622. 34. , and Niedermann, G. (1999) Giant protease with potential to substitute for some functions of the proteasome. Science 283, 978–981.

The introduction of proteasome inhibitors to cellular studies enabled the demonstration of the dominant role of this protease in cellular protein turnover and its involvement in the generation of class I ligands (6). Currently, there are four kinds of commonly used proteasome inhibitors, all of which, through different mechanisms, base their activity on the modification of the gamma oxygen (Oγ) on the N-terminal, active residue of Thr in one or more of the catalytic β-subunits (for a review on the mechanisms of the inhibitors, see ref.

Stock solutions of inhibitors as outlined in Table 2. 3. 1. Ruling Out Peptide Contamination Any study of the presentation of exogenous Ags on MHC-I must rule out peptide contamination in the Ag preparation. Despite attempts to remove peptide from Ag preparations by gel filtration, centrifugation, or other methods, it is possible that peptide contamination that is undetectable biochemically may still be present. Such peptide contamination will dramatically affect the results, and may be misleading.

Download PDF sample

Antigen Processing and Presentation Protocols (Methods in Molecular Biology Vol 156) by Solheim J.C.


by Paul
4.3

Rated 4.33 of 5 – based on 19 votes