By Mark A. Herbert (ed.), Derek W. Hood (ed.), E. Richard Moxon (ed.)
Because the moment best bacterial reason behind community-acquired pneumonia, H. influenzae money owed for lots of hundreds of thousands of deaths consistent with yr around the globe, and is a first-rate candidate for the id of ambitions which can result in the improvement of novel vaccines and antibiotics. In Haemophilus influenzae Protocols, best study scientists and infectious sickness experts aspect in a with ease reproducible layout the most important molecular and immunological innovations for exploring the pathogenicity of this crucial bacterium. defined with step by step directions to make sure powerful and winning experimental effects, the suggestions conceal plasmid research, proteomics, genomics, DNA array know-how, gene expression, mutagenesis (transposon and nontransposon), and structural research. The antibody ideas offered contain IVIAT, ELISA, and opsonophagocytosis assays. additionally integrated are studies of the pathogenesis of NTHi and Hib, equipment for operating with the organism, tradition and garage stipulations, molecular prognosis, and platforms for exploring pathogenesis in either in vitro and animal models.
Comprehensive and hugely functional, Haemophilus influenzae Protocols bargains a special choice of strong molecular equipment that remove darkness from not just how the bacterium explanations affliction, but additionally how top to advance much-needed new vaccines and antibiotics opposed to it.
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Extra info for Haemophilus influenzae Protocols
St. Geme, J. , III and Falkow, S. (1990) Haemophilus influenzae adheres to and enters cultured human epithelial cells. Infect. Immun. 58, 4036–4044. 79. Barenkamp, S. J. and Leininger, E. (1992) Cloning, expression, and DNA sequence analysis of genes encoding nontypeable Haemophilus influenzae highmolecular-weight proteins related to filamentous hemagglutinin of Bordatella pertussis. Infect. Immun. 60, 1302–1313. 80. Barenkamp, S. J. and Bodor, F. F. (1990) Development of serum bactericidal activity following nontypeable Haemophilus influenzae acute otitis media.
The pilus rods are composed of polymerized pilin proteins, which show slight interstrain variability in migration by polyacrylamide gel electrophoresis (PAGE) (67). At least 16 pili are expressed per bacterial cell (66) and are distributed in a peritrichous manner (68). Pili appear to mediate bacterial adherence to mucosal surfaces and hence facilitate respiratory tract colonization. Anderson and co-workers observed that a piliated H. influenzae strain showed stronger adherence to buccal epithelial cells and was more effective in colonizing rats following intranasal inoculation than its nonpiliated variant (69).
59. Denny, F. W. (1974) Effect of a toxin produced by Haemophilus influenzae on ciliated respiratory epithelium. J. Infect. Dis. 129, 93–100. 60. Johnson, A. P. and Inzana, T. J. (1986) Loss of ciliary activity in organ cultures of rat trachea treated with lipo-oligosaccharide from Haemophilus influenzae. J. Med. Microbiol. 22, 265–268. 61. , Apicella, M. , and Lim, D. (1995) Quantitation and biological properties of released and cell-bound forms of lipooligosaccharides from nontypeable Haemophilus influenzae.
Haemophilus influenzae Protocols by Mark A. Herbert (ed.), Derek W. Hood (ed.), E. Richard Moxon (ed.)